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KMID : 0665220080210030269
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Korean Journal of Food and Nutrition
2008 Volume.21 No. 3 p.269 ~ p.274
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Studies on the Effects of Water Extract from Mixture of Pine Needles, Sedum sarmentosum Bunge, Hijkiaorme, Buckwheat and Perlla Leaves on the Immune Function Activation
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Ryu Hye-Sook
Kim Hyun-Sook
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Abstract
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Plants have long been used as a food source in Korea. In this study, we investigated the combined immunomodulative effects of a water extract mixture of(pine needles, Sedum sarmentosum Bunge, hijkiaorme, buckwheat and Peril a leaves) on Balb/c mice weeks old. The mice were fed a chow diet ad libitum and the plant extract was orally administered every other day for four weeks at two different concentrations(50 and 500 mg/kg BW). After preparing the single-cell suspension, splenocyte proliferation was determined by the MTT(3-[4,5-di-methylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. After 48hrs of incubation with the mitogens(ConA or LPS) splenocyte from the mice groups administered 50 and 500 mg/kg BW of the plant extract showed a significant increased in proliferation compared to the control group. A hemolytic plague forming cell assay was used to indicate antibody production against sheep red blood cells(SRBC). The number of antibody-secreting cells T-dependent antigen. The result of this study suggest that supplementation with this plant extract may regulate immune function by increasing splenocyte proliferation and the number of plaque forming cells.
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KEYWORD
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mixture, splenocyte proliferation, SRBC, immune
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